Our data indicate that the CRISPR/dCas9 DNA-targeting platform can be used in plants as a functional genomics tool and for biotechnological applications. Thus, our results suggest that the synthetic transcriptional repressor (dCas9:SRDX) and activators (dCas9:EDLL and dCas9:TAD) can be used as endogenous transcription factors to repress or activate transcription of an endogenous genomic target. Male sterility in Arabidopsis induced by overexpression of a MYC5SRDX chimeric repressor Author: Pablo Figueroa, John Browse Source: plant journal 2015 v.81 no.6 pp. Further, the dCas9:SRDX-mediated transcriptional repression of an endogenous gene. Our data demonstrate that dCas9 fusion with the EDLL activation domain (dCas9:EDLL) and the TAL activation domain (dCas9:TAD), guided by gRNAs complementary to selected promoter elements, induce strong transcriptional activation on Bs3::uidA targets in plant cells. To generate a transcriptional repressor, we fused the dCas9 C-terminus with the SRDX repression domain. To generate transcriptional activators, we fused the dCas9 C-terminus with the activation domains of EDLL and TAL effectors. Here, we modified this DNA-targeting platform for targeted transcriptional regulation in planta by developing chimeric dCas9-based transcriptional activators and repressors. Estradiol-induced expression of ATML1-SRDX in the seedlings decreased transcript levels of several epidermis-related genes. 127 a synthetic target promoter, POpto, integrating the binding. A recent study used the catalytically inactive Cas9 (dCas9) protein combined with guide-RNAs (gRNAs) as a DNA-targeting platform to modulate gene expression in bacterial, yeast, and human cells. To further assess the roles of ATML1 and its homologs in epidermal cell differentiation, I generated transgenic plants expressing ATML1 fused with a transcriptional repressor sequence (ATML1-SRDX). 126 PIF6, ii) a constitutively expressed blue light-repressor module SRDX-EL222, and iii). The type II CRISPR/Cas system has been adapted for genome editing in many cell types and organisms. Bacteria and archaea use clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) regulatory systems for adaptive molecular immunity against foreign nucleic acids introduced by invading phages and conjugative plasmids. Summary: Targeted genomic regulation is a powerful approach to accelerate trait discovery and development in agricultural biotechnology.
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